MITOCHONDRIAL AND CYTOPLASMIC RIBOSOMES FROM TETRAHYMENA PYRIFORMIS
نویسندگان
چکیده
منابع مشابه
Mitochondrial and Cytoplasmic Ribosomes from Tetrahymena Pyriformis
Mitochondrial and cytoplasmic ribosomes from Tetrahymena pyriformis have been isolated and studied by the techniques of polyacrylamide gel electrophoresis and electron microscopy used in conjunction. Although the two ribosome types show the same coefficient of sedimentation (80S) in sucrose gradients, they can be distinguished by gel electrophoresis: mitoribosomes migrate in a single band, cons...
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We have examined the fatty acid composition of the mitochondrial membranes in three strains of Tetrahymena pyriformis. All three had similar components and exhibited large amounts of unsaturated fatty acids. The cytoplasmic mutant, CA-10, which has a slower growth rate and unusual membrane morphology, had a slightly higher amount of iso-acids but was otherwise similar to the other strains in fa...
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The ribosomes extracted from the mitochondria of the ciliate, Paramecium aurelia, have been shown to sediment at 80S in sucrose gradients. The cytoplasmic ribosomes also sediment at 80S but can be distinguished from their mitochondrial counterparts by a number of criteria. Lowering of the Mg++ concentration, addition of EDTA, or high KCl concentrations results in the dissociation of the cytopla...
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Electron microscopic radioautography has been used to study the synthesis of mitochondrial DNA after incorporation of thymidine-(3)H by cultures in logarithmic phase of Tetrahymena pyriformis during periods ranging from 15 min to 12 hr. The great majority of silver grains are distributed over the macronuclei, the micronuclei, and the mitochondria. The intensity of the label over the entire mito...
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Cytoplasmic D N A is known in a number of cell types. Hoff-JCrgenson and Zeuthen (7), using a microbial bioassay method, reported the presence of cytoplasmic D N A in frog eggs. Later, Hoff-J~6rgenson (8) and Elson et al. (4) indicated that sea urchin eggs contained a D N A reserve in the cytoplasm. Using autoradiographs of whole cells, Scherbaum (21) described cytoplasmic labeling in Ha-thymid...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1974
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.60.3.628